Micropropagation Harsh Natural Environment

Question: Discuss about the Micropropagationfor Harsh Natural Environment. Answer: Introduction Micropropagation is an activity that involves scientist coming up with an exact copy of an individual plant. Also known as clonal propagation, micropropagation improves yields; achieve better traits like the color as well as creating resistance against pests. A researcher, Sean developed an interest in rapid multiplication of a particular stock plant. However, the plantlets did not grow roots and finally died due to the harsh natural environment The reason for the death of explant was the season in which the researcher obtained the explant. Sean may have cultured the explant in harsh conditions. The shoots and root cells are in a continuous state of growth. As a result, buds are dormant as compared to shoots and roots as the season fall from spring, summer and eventually winter (Beck,2010). The tissue of the explant still exists in physiological dormancy until the cells meet dormancy requirements. Root cells require an optimum or room temperature. The plantlets also failed to get enough roots due to insufficient light. Light is an essential aspect, especially with leaf cuttings or stem cuttings. It is vital so that the plant can photosynthesize and create carbohydrates that lead to the development of roots. Furthermore, too much light can result in harsh conditions that dry up the plant. The temperature will also have an effect on the formation of roots. It is imperative to have an air temperature of about 65F to ensure the successful development of roots (Naz, Anis Alatar, 2016). Furthermore, the roots will develop faster when the bottom heat of the rooting medium is at a temperature that is 10F warmer. Also, Sean may have used either too big or too small explant which is not easy to culture. The culture solution has additional components which may be difficult to sustain a big explant with a significant content of nutrients. Just to add, the explant has different morphogenetic potential depending on whether the part was from roots, stem or the shoot (DeberghZimmermann,2012). In the current context, the plantlets failed to grow due to a deficiency of disease resilience. This is a common occurrence that arises when a monoculture is developed through micropropagation. It meant that the plant could easily be infected, leading to its death. The plant can also die off when it produces metabolic chemicals that can kill or stunt the explant. In some cases, the cultivars or plants may not develop after undergoing tissue culture. It can depend on the kind of explant that has been using during the initial phase, or it can arise due to the age of the propagule line or cell. In some cases, some plants cannot successfully be tissue cultured (Souza, Nietsche, Xavier, Costa, Pereira Santos, 2016). It arises when the best medium for growth is unknown. In this case, the plantlets will not get sufficient roots. Sean should sterilize the tissues surfaces. Sterilization assists to reduce contamination of the underlying cells by fungi and bacterial infections. Secondly, the researcher should provide oxygen and optimum temperature through proper incubation(DeberghZimmermann,2012). Moreover, Sean should provide an optimum rate of gas and pressure. Additionally, the researcher should transplant the plantlets in the greenhouse since the seedlings are sensitive to the harsh environment. References Beck, C. (2010). An introduction to plant structure and development (2nd ed.). Boston: Cambridge University Press. Debergh, P Zimmerman, H. (2012) Micropropagation: Technology and Application. Berlin: Springer Science Naz, R., Anis, M., Alatar, A. A. (2016). ISSR marker?based detection of genomic stability in Cassia occidentalis L. plantlets derived from somatic embryogenesis.Engineering in Life Sciences,16(1), 17-24. Souza, G. L., Nietsche, S., Xavier, A. A., Costa, M. R., Pereira, M. C., Santos, M. A. (2016). Triple combinations with PGPB stimulate plant growth in micropropagated banana plantlets.Applied Soil Ecology,103, 31-35.